Biology of Sport
pISSN 0860-021X    eISSN 2083-1862
Archival Issues
Volume 34, 2017
Volume 33, 2016
Volume 32, 2015
Volume 31, 2014
Volume 30, 2013
Volume 29, 2012
Volume 28, 2011
Volume 27, 2010
Volume 26, 2009
Volume 25, 2008
Volume 24, 2007
Volume 23, 2006
Volume 22, 2005
Volume 21, 2004
Volume 20, 2003
Archival Issues 1984-1998
Search
Newsletter
Information for Authors
Special Information
 » 
Journal Abstract
 
siRNA-induced silencing of hypoxia-inducible factor 3α (HIF3α) increases endurance capacity in rats
Svitlana B. Drozdovska, Bronislav L. Gavenauskas, Tetyana I Drevytska , Valentina I Nosar, Vasiliy S Nagibin, Irina M. Mankovska , Victor E Dosenko
Biol Sport 2016; 33(2):99-106
ICID: 1196509
Article type: Original article
IC™ Value: 10.00
Abstract provided by Publisher
 
Molecular mechanisms of adaptation to exercise despite a large number of studies remain unclear. One of the crucial factors in this process is hypoxia inducible factor (HIF) that regulates transcription of many target genes encoding proteins that are implicated in molecular adaptation to hypoxia. Experiments were conducted on 24 adult male Fisher rats. Real-time PCR analysis was performed for quantitative evaluation of Hif3α, Igf1, Glut-4 and Pdk-1 in m. gastrocnemius, m. soleus, in lung and heart tissues. Mitochondrial respiratory function and electron microscopy were performed. Knockdown of Hif3α using siRNA increases time of swimming to exhaustion by 1.5 times. Level of mitochondrial NAD- and FAD-dependent oxidative pathways is decreased, however efficiency of phosphorylation is increased after Hif3α siRNA treatment. Expression of HIF target genes in muscles was not changed significantly, except for increasing of Pdk-1 expression in m. soleus by 2.1 times. More prominent changes were estimated in lung and heart: Igf1 gene expression was increased by 32.5 and 37.5 times correspondingly. Glut4 gene expression in lungs was increased from undetected level till 0.3 rel. units and by 84.2 times in heart. Level of Pdk1 gene expression was increased by 249.2 in lungs and by 35.1 times in hearts, correspondingly. Some destructive changes in muscle tissue were detected in animals with siRNA-inducing silencing of Hif3α.

ICID 1196509

DOI 10.5604/20831862.1196509
PMID 27274101 - click here to show this article in PubMed
 
FULL TEXT 1579 KB


Related articles
  • in IndexCopernicus™
         hypoxia-inducible factor 3α [0 related records]
         endurance capacity [0 related records]
         RNA Interference [64 related records]

  • Related articlesin PubMed database
  • in PubMed database [ related records]


  •  

    Copyright © Biology of Sport  2017
    Page created by Index Copernicus Ltd. All Rights reserved.