Plasma lipid peroxidation, blood GSH concentration and erythrocyte antioxidant enzymes in menstruating females with ovulatory and anovulatory cycles compared with males G Lutosławska, J Tkaczyk, E Hübner-Woźniak, B Panczenko-Kresowska, E Skierska, AK Gajewski Biol Sport 2003; 20(4):331-341 ICID: 891990
Article type: Original article
IC™ Value: 10.26
Abstract provided by Publisher
This study was undertaken to evaluate plasma TBARS and blood GSH concentration and erythrocyte antioxidant enzymes (glutathione peroxidase, catalase and superoxide dismutase) in active, regularly menstruating female physical education students with ovulatory and anovulatory menstrual cycles and in their male counterparts. A total of 27 subjects (12 males and 15 females) volunteered to participate in the study. All females were regularly menstruating with cycle length between 26-31 days. Plasma progesterone and 17--estradiol concentrations were assayed during the 7th-9th and 22nd-25th day of the menstrual cycle. Women with plasma progesterone concentration exceeding 19 nmol•l-1 during the 22nd-25th day were referred to as ovulatory (Group OV; n=7). Women without a peak plasma progesterone concentration were referred to as anovulatory (Group AN; n=8). Blood from male subjects was withdrawn twice - two weeks apart, at their convenience. It was found that the menstrual cycle phases did not affect plasma TBARS and blood glutathione concentration and erythrocyte GPX, CAT and SOD activity. However, erythrocyte GPX activity either in ovulatory or anovulatory women was by about 30% higher than in male subjects. Erythrocyte SOD activity in ovulatory women both in follicular and luteal phase of the menstrual cycle (1557 U/g Hb and 1394.6 U/g Hb, respectively) was markedly lower than in men (1951.8 and 1937.7 U/g Hb for blood sampling I and II, respectively). In contrast, erythrocyte SOD activity in anovulatory women (1855.5 U/g Hb and 1745.7 U/g Hb in the follicular and luteal phases, respectively) was similar to that found in men. The above data indicated that erythrocyte GPX and SOD activities are sensitive to plasma ovarian hormone concentration. In addition, they suggested that due to higher erythrocyte GPX activity females even with anovulatory menstrual cycles are protected better than males against hydrogen peroxide action. However, lower superoxide production and consequent lower erythrocyte SOD activity was solely observed in females with ovulatory menstrual cycles and normal ovarian hormone plasma levels.